lb agar plates with gentamicin 7  (Teknova)

Journal: Journal of Clinical Microbiology

Article Title: Update on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Identification of Filamentous Fungi

doi: 10.1128/JCM.01263-20

Figure Lengend Snippet: Growth conditions for filamentous fungi generating the most distinguishable peaks a

Article Snippet: Nevertheless, if the extraction protocol differs from the one used to build the reference library, different spectra might be obtained, and the fungi might not be correctly identified. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Growth conditions Method for harvest of material Extraction step(s) Species (no. of isolates) Reference 72 h at 27°C on Sabouraud gentamicin-chloramphenicol agar plates Harvested by scraping with a sterile plastic device FA extraction Aspergillus spp. (43), Penicillium spp. (16), Scedosporium spp. (18), Fusarium spp. (11), other (68) 1 24 h at 27°C in Sabouraud broth 10-min centrifugation at 13,000 × g Pellet washed 3 times with 1 ml of sterile water and suspended with 300 μl of HPLC sterile water and 900 μl of anhydrous ethyl alcohol; FA extraction Aspergillus spp. (43), Penicillium spp. (16), Scedosporium spp. (18), Fusarium spp. (11), other (68) 1 72 h at 27°C on Sabouraud gentamicin-chloramphenicol agar plates Harvested by scraping with a sterile plastic device Hydroalcoholic suspension of fungal material lysed by 3 cycles of microbeads with a FastPrep-24 instrument; FA extraction Aspergillus spp. (43), Penicillium spp. (16), Scedosporium spp. (18), Fusarium spp. (11), other (68) 1 72 h at 27°C on Sabouraud dextrose agar with antibiotics FA extraction Pseudallescheria-Scedosporium species complex 8 72 h at 27°C on malt agar TFA extraction Pseudallescheria-Scedosporium species complex 8 72 h at 27°C on malt agar Surfaces scraped using a sterile scalpel TFA extraction Clinical Fusarium isolates 9 Open in a separate window a Abbreviations: FA, formic acid; HPLC, high-performance liquid chromatography; TFA, trifluoroacetic acid.

Techniques: Centrifugation

Journal: Immunity

Article Title: Structure of CC Chemokine Receptor 5 with a Potent Chemokine Antagonist Reveals Mechanisms of Chemokine Recognition and Molecular Mimicry by HIV

doi: 10.1016/j.immuni.2017.05.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: LB Agar plates with Gentamicin 7, Kanamycin 50, Tetracycline 10 Bluo gal 100, IPTG 100mm plates , Teknova , Cat# L1919.

Techniques: Expressing, Recombinant, Protease Inhibitor, Transfection, Mutagenesis, Cell Culture, Software, Molecular Weight